A sandwich electrochemical immunosensor based on antibody functionalized-silver nanoparticles (Ab-Ag NPs) for the detection of dengue biomarker protein NS1
By Awan, Maryam; Rauf, Sajid; Abbas, Azhar; Nawaz, Mian Hasnain; Yang, Changping; Shahid, Shakir Ahmad; Amin, Naima; Hayat, Akhtar
Published in Journal of Molecular Liquids
Journal of Molecular Liquids
2020
Abstract
Dengue, a viral disease caused by a flavivirus belonging to family. Flaviviridae, is a serious threat to human health in tropical and subtropical regions worldwide. Herein, we design a sandwich-type immunosensor based on a nano-probe maintaining antibody functionalized?silver-nanoparticles (Ab-Ag NPs) as a trace tag for simple, sensitive, and selective detection of dengue biomarker NS1. Diazonium assisted immobilization of antibody on graphite pencil electrodes provided a selective and specific platform for analyte (NS1) recognition, sandwiched by Ab-Ag NPs. Well defined and sharp electrochemical signals by Ag NPs, characteristics of their oxidation, probed the specific detection of NS1. A proportional increase in the faradic current for the oxidation of Ag NPs is observed with an increase in analyte NS1 concentration. This sandwiched based immunosensor facilitated to achieve a linear range of 3