Polypyrrole/Carbon Nanotubes/Lactate Oxidase Nanobiocomposite Film based Modified Stainless Steel Electrode Lactate biosensor
By Bhavna H. Meshram and Ritu P. Mahore and Priyanka D. Virutkar and Subhash B. Kondawar
Published in Procedia Materials Science
2015
Abstract
Highly sensitive electrochemical lactate biosensor was constructed by one-step preparation route of amperometric enzyme electrode based on carbon nanotubes (CNT) and biocatalyst within an electropolymerized polypyrrole (PPy) film electrodeposited on stainless steel electrode. The nanobiocomposite film was electrochemically synthesized by electro-oxidation of pyrrole in a neutral pH solution containing appropriate amounts of lactate oxidase (LOD) and functionalized multi-walled carbon nanotubes (c-MWCNT). The nanobiocomposite film was characterized by FTIR, \SEM\ and EIS. The incorporation of \CNT\ facilitated high enzyme loading, increase in lifetime, stability and fast response time of the enzyme electrode. In present work artificial sweat was used as a non-invasive analyte for detection of lactate. When PPy/c-MWCNT/LOD modified electrode tested for lactate in artificial sweat, the apparent Michaelis