Amperometric mediatorless hydrogen peroxide sensor with horseradish peroxidase encapsulated in peptide nanotubes
By Hamid Feyzizarnagh and Byung-Wook Park and Lohit Sharma and Michelle M. Patania and Do Young Yoon and Dong-Shik Kim
Published in Sensing and Bio-Sensing Research
2016
Abstract
A mediatorless sensor with horseradish peroxidase (HRP) enzymes encapsulated inside peptide nanotubes (PNTs) has been proposed for amperometric detection of hydrogen peroxide. \PNTs\ not only encapsulate the enzymes to retain their activity and stability, but also can provide direct electron transfer between an electrode and the electroactive sites of \HRP\ without mediators. Experimental results were compared with hydroquinone (HQ)-mediated electron transfer results. The PNT/HRP sensor produced a current signal comparable to the HQ/HRP sensor in the entire range of hydrogen peroxide concentrations (0