Application of mixed self-assembled monolayers (Mixed SAMs) for nucleic acid detection
By Zehra Banu Bahsi Oral
Published in Measurement
2016
Abstract
Mixed self-assembled monolayers (Mixed SAMs) consisting of 8-Ferrocenyl-1-octanethiol and 6-Mercapto-1-hexanol (FcOT:MCH) with probe \PNA\ on gold electrodes were fabricated by using two-step after the optimization of immobilization temperature of FcOT:MCH SAMs. Using \AC\ voltammetry, a novel nucleic acid detection platform, with mixed SAMs, was proposed. A negative formal potential shift was observed after complementary ssDNA hybridization while there was no significant difference after non-complementary ssDNA hybridization. Compatible results were obtained with the measurement of formal potential differences between mixed \SAMs\ and target \DNA\ (complementary and non-complementary DNA) in different target \DNA\ concentrations. The formal potential difference between mixed \SAMs\ immobilization and complementary ssDNA hybridization was measured in different ionic strength concentrations.