A novel sensitive amperometric choline biosensor based on multiwalled carbon nanotubes and gold nanoparticles
By Magar, Hend Samy; Ghica, Mariana Emilia; Abbas, Mohammed Nooredeen; Brett, Christopher M. A.
Published in Talanta
2017
Abstract
Abstract A novel amperometric biosensor for choline determination has been developed, exploiting the electrocatalytic properties of multiwalled carbon nanotubes (MWCNT) and gold nanoparticles (GNP). Chitosan (Chit), a natural biocompatible polymer, was used to disperse CNT, then Chit-MWCNT was dropped on the surface of a glassy carbon electrode (GCE), followed by GNP; finally, choline oxidase (ChOx) was immobilized by glutaraldehyde crosslinking. The ChOx/(GNP)4/MWCNT/GCE exhibited linear response to choline from 3 to 120