Alginate copper oxide nano-biocomposite as a novel material for amperometric glucose biosensing
By Buk, Vuslat; Emregul, Emel; Emregul, Kaan Cebesoy
Published in Materials Science and Engineering: C
2017
Abstract
Abstract A novel amperometric glucose biosensor based on alginate-CuO nano-biocomposite and glucose oxidase (GOD) film was developed and characterized. The properties of the alginate-CuO-GOD film were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Amperometric measurements were employed to characterize the analytical performance of the biosensor. Several parameters including amount of alginate, concentration of {GOD} and cross-linkers, amount of CuO nanoparticles, and effect of pH were studied and optimized. Under optimal conditions, the developed alginate-CuO-GOD biosensor was shown to have two linear ranges; from 0.04 mM to 3 mM (with a correlation coefficient of 0.9996 and the sensitivity of 30.443 ?A mM? 1 cm? 2) and from 4 mM to 35 mM (with a correlation coefficient of 0.9994 and the sensitivity of 7.205 ?A mM? 1 cm? 2). The overall detection limit was estimated to be 1.6 ?M (signal-to-noise ratio of 3) and the Km value of 2.82 mM. The biosensor exhibited rather good performance with long-term stability (remainder of activity is 78% after 15 days) and significant specificity for glucose when compared to possible interfering molecules such as ascorbic acid, uric acid and acetaminophen.